بررسی، استخراج و ارزیابی شیمیایی رنگدانه قرمز آنتوسیانین در برگ پاییزی گیاه نادینا دامستیکا (بربریداسئا)

نوع مقاله : مقاله پژوهشی

نویسنده

گروه صنایع آلی و دارویی، پژوهشکده فناوری‌های شیمیایی، سازمان پژوهش‌های علمی و صنعتی ایران، تهران، صندوق پستی: 33535111

چکیده

مطالعات نشان داده است که بروز رنگ‌های زرد و قرمز در برگ‌های پاییزی به ترتیب ناشی از رنگدانه‌های کاروتنوئید و آنتوسیانین است. آنتوسیانین‌ها در گیاهان خاصی بسته به گونه و طبیعت آنها، در طول پیری برگ، در فصل پاییز با وجود آفتاب کافی اما کاهش دما و طول روز سنتز می‌شوند. در این پژوهش، پدیدار شدن رنگدانه قرمز فام آنتوسیانین در برگ پاییزی گیاه نادینا دامستیکا بربریداسئا مورد بررسی و آنالیز شیمیایی قرار گرفت. برگ قرمز پاییزی از گیاهان در محیط تهران جمع‌آوری، خشک و آسیاب گردید. آنگاه رنگدانه‌های موجود در آن به وسیله حلال اتانل به طور کامل استخراج شد. رنگدانه‌های کاروتنوئیدی و آنتوسیانینی موجود در محلول استخراجی با استفاده از حلال هگزان نرمال از هم جدا شدند. رنگدانه غالب آنتوسیانینی مورد آزمایش‌های طیف‌سنجی نوری مرئی، کروماتوگرافی لایه نازک، رنگ‌سنجی و کروماتوگرافی مایع با کارایی بالا (HPLC) قرار گرفت. نتایج بیانگر وجود ترکیبات آنتوسیانینی، شامل دلفینیدین، سیانیدین، پتونیدین، پلارگونیدین و مالویدین با مقادیر متفاوت در برگ پاییزی گیاه نادینا است که موجب فام قرمز غالب و جذاب آن شده است.

کلیدواژه‌ها


عنوان مقاله [English]

Investigation, Extraction and Chemical Evaluation of Anthocyanin Red Pigment of Nadina Domestica (berberidaceae) shrub in autumn leaves

نویسنده [English]

  • Zaker Bahreini
Department of Chemical Technologies, Iranian Research Organization for Science and Technology, P. O Box: 3353511, Tehran, Iran
چکیده [English]

Studies have proved that the appearance of yellow and red colors in autumn leaves results from carotenoid and anthocyanin pigments, respectively. Anthocyanins are synthesized in certain plants, depending on species composition and phenology, during leaf senescence, in the autumn season at enough sunshine but decreased temperature and day length. In this study, the accumulation of red anthocyanin pigment in Nadina domestica (berberidaceae) shrub autumn leaves was investigated. Leaves of the mentioned shrub were collected from Tehran environment, fully dried, and ground. The anthocyanins and carotenoid content of the powder were extracted using ethanol as a solvent. These pigments were separated by n-hexane solvent. Anthocyanin components were characterized by the following methods: UV-visible spectrophotometry, thin-layer chromatography (TLC), colorimetry parameters (CIE LAB), and high-performance liquid chromatography (HPLC). The results showed that anthocyanin compounds, including Cyanidin, Delphinidin, Petunidin, Plargonidin, and Malvinidin, are the major and attractive red color anthocyanins of the leaves extract.

کلیدواژه‌ها [English]

  • Anthocyanin
  • Plant pigments
  • Autumn leaves
  • Nadina shrub
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