بررسی رنگبری متیلن‌بلو توسط بیوپلیمرپلی‌هیدروکسی‌بوتیرات و باکتری‌های جداسازی‌ شده از پساب نساجی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشکده مهندسی نساجی، دانشگاه یزد، یزد، ایران، صندوق‌پستی: 741-89195

2 گروه زیست‌شناسی، دانشگاه یزد، یزد، ایران، صندوق‌پستی: 741-89195

10.30509/jcst.2026.167761.1281

چکیده

آلودگی پساب‌های نساجی با رنگزاهای صنعتی یکی از تهدیدهای جدی محیط‌زیست است و به‌دلیل مقاومت شیمیایی بالای این رنگزاها، توسعه روش‌های کارآمد برای حذف آن‌ها اهمیت بالایی دارد. در این پژوهش، دو گونه باکتری جدید (Megaterium SH3 OR635822, Bacillus cereus SH4 OR636055) و همچنین فیلم تولیدی پلی‌هیدروکسی‌بوتیرات (PHB) به‌عنوان جاذب‌های زیستی برای حذف رنگزای کاتیونی متیلن بلو مورد بررسی قرار گرفتند. نتایج نشان دادند که سویه SH4 در شرایط بهینه (غلظت رنگزا mg/l 100، مدت زمان تماس 72 ساعت و 7= pH) بیشترین بازده‌‌‌ حذف رنگزا را به میزان 28/45 درصد نشان داد، در‌حالی‌که فیلم PHB در شرایط بهینه خود (غلظت رنگزا  mg/l 100، مدت زمان تماس 60 دقیقه و10=pH) توانست 50/81 درصد از ماده رنگزا را حذف کند. تحلیل‌های ایزوترم، سینتیک و ترمودینامیک نشان دادند که مدل شبه‌مرتبه دوم و ایزوترم لانگمویر بهترین تطابق را با داده‌های حاصل از فرایند رنگبری دارند و فرایند جذب به‌صورت خودبه‌خودی و گرماگیر انجام می‌شود. این مطالعه نوآورانه، پتانسیل بالای باکتری‌های جدید و فیلم تولیدی PHB به‌عنوان جاذب‌های زیستی کارآمد برای تصفیه پایدار پساب‌های رنگی را نشان می‌دهد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigation of Methylene Blue Decolorization Using Polyhydroxybutyrate Biopolymer and Bacteria Isolated from Textile Wastewater

نویسندگان [English]

  • Zahra Shahi 1
  • Mohammad Khajeh Mehrizi 1
  • Maryam Sadat Mirbagheri Firoozabad 2
1 Department of Textile Engineering, Yazd University, P.O. Box: 89195-741, Yazd, Iran
2 Department of Biology, Yazd University, P.O. Box: 89195-741, Yazd, Iran
چکیده [English]

Contamination of textile wastewater with industrial dyes poses a serious environmental threat, and due to the high chemical stability of these dyes, developing efficient removal methods is essential. In this study, two novel bacterial strains (Megaterium SH3 OR635822, and Bacillus cereus SH4 OR636055) and a produced polyhydroxybutyrate (PHB) biofilm were evaluated as bioadsorbents for the removal of the cationic dye methylene blue. Results showed that strain SH4 exhibited the highest dye removal efficiency of 28.45 % under optimal conditions (dye concentration= 100 mg/L, contact time =72 h, pH =7), whereas the PHB biofilm reached 50.81 % under its optimal conditions (dye concentration =100 mg/L, contact time= 60 min, pH =10). Isotherm, kinetic, and thermodynamic analyses indicated that the pseudo-second-order kinetic model and Langmuir isotherm provided the best fit to the experimental data, and the adsorption process was spontaneous and endothermic. This innovative study highlights the high potential of novel bacterial strains and the produced PHB biofilm as efficient bioadsorbents for sustainable treatment of colored wastewater.

کلیدواژه‌ها [English]

  • Textile wastewater
  • Decolorization
  • Methylene blue
  • Bacteria
  • PHB biofilm

مراجع

  1. Sarkheil H, Noormohammadi F, Rezaei AR, Borujeni MK. Dye pollution removal from mining and industrial wastewaters by using Chitson nanoparticles. Int Conf Agric Environ Biol Sci. 2014;4:1437-1442.
  2. Al-Tohamy R, Ali SS, Li F, Okasha KM, Mahmoud YA-G, Elsamahy T, et al. A critical review on the treatment of dye-containing wastewater: Ecotoxicological and health concerns of textile dyes and possible remediation approaches for environmental safety. Ecotoxicol Environ Saf. 2022; 231:113160. https://doi.org/10.1016/j.ecoenv. 2021.113160.
  3. McMullan G, Meehan C, Conneely A, Kirby N, Robinson T, Nigam P, et al. Microbial decolourisation and degradation of textile dyes. Appl Microbiol Biotechnol. 2001;56:81–87. https://doi.org/10.1007/s002530000587.
  4. Alsukaibi AKD. Various approaches for the detoxification of toxic dyes in wastewater. Processes. 2022;10(10):1968. https://doi.org/10.3390/pr10101968
  5. Osorio-Aguilar DM, Saldarriaga-Noreña HA, Murillo-Tovar MA, Vergara-Sánchez J, Ramírez-Aparicio J, Magallón-Cacho L, et al. Adsorption and photocatalytic degradation of methylene blue in carbon nanotubes: a review with bibliometric analysis. Catal. 2023;13:1480. https://doi.org/ 10.3390/catal13121480
  6. Pratama SA, Purnomo AS, Asranudin A. Methylene blue removal using alginate-PVA-Pseudomonas aeruginosa bio-composite: Kinetics and biodegradation studies. Biotechnol Rep. 2025;e00906. https://doi.org/10.1016/j.btre. 2025.e00906
  7. Wu K, Shi M, Pan X, Zhang J, Zhang X, Shen T, et al. Decolourization and biodegradation of methylene blue dye by a ligninolytic enzyme-producing Bacillus thuringiensis: Degradation products and pathway. Enzyme Microb Technol. 2022;156:109999. https://doi.org/10.1016/j.enzmictec. 2022. 109999.
  8. Heitmann AP, Rocha IC, de Souza PP, Oliveira LCA, de O. Patrício PS. Photoactivation of a biodegradable polymer (PHB): Generation of radicals for pollutants oxidation. Catal Today. 2020;344:xx. https://doi.org/10.1016/j.cattod.2018. 12. 024.
  9. Shahi Z, Khajeh Mehrizi M, Mirbagheri Firoozabad MS. Biopolymers in the Textile Industry. Springer; 2020. https://doi.org/10.1007/978-981-97-0684-6.
  10. Acharjee SA, Bharali P, Gogoi B, Sorhie V, Walling B, Alemtoshi. PHA-Based Bioplastic: a Potential Alternative to Address Microplastic Pollution. Water Air Soil Pollut. 2023;234:xx. https://doi.org/10.1007/s11270-022-06029-2.
  11. Montanheiro TLA, Cristóvan FH, Machado JPB, Tada DB, Durán N, Lemes AP. Effect of MWCNT functionalization on thermal and electrical properties of PHBV/MWCNT nanocomposites. J Mater Res. 2015;30:xx. https://doi.org/10. 1557/jmr.2014.303.
  12. Choonut A, Prasertsan P, Klomklao S, Sangkharak K. An environmentally friendly process for textile wastewater treatment with a medium-chain-length polyhydroxyalkanoate film. J Polym Environ. 2021;29:3335-3346. https://doi.org/ 10.1007/s10924-021-02121-6.
  13. Etezad SM, Sadeghi-Kiakhani M. Decolorization of malachite green dye solution by bacterial biodegradation. Prog Color Colorants Coat. 2021;14:79-87. https://doi.org/10.30509/ PCCC.2021.81670.
  14. Darvishi L, Ebrahimi A, Mahzounieh MR. Isolation and investigation of the effect of soil and textile wastewater bacteria on decolorization of industrial dyes. J Ilam Univ Med Sci. 2019;27(1):32-43. (In Persian).
  15. Saber Tahan M, Farazmand A, Pourbabaee A. The removal of dye colours from textile wastewater factories using mixed thermophilic bacterial consortium. NCMBJ. 2017;7(28):25-32. (In Persian).
  16. Shahi Z, Khajeh Mehrizi M, Mirbagheri Firoozabad MS. Decolorization of textile dye by isolated bacterial strains from textile waste and their ability to produce polyhydroxy-alkanoate. Prog Color Colorants Coat. 2024; 18 (1):99-111. https://doi.org/10.30509/PCCC.2024.167299. 1292.
  17. Shahi Z, Khajeh Mehrizi M, Mirbagheri Firoozabad  MS, Adeel  Sh. Biodecolorization of textile dye using novel bacterial, film-based, and immobilized nanofiber systems for environmental wastewater treatment: a comparative study. J Environ Chem Eng. 2026; 14(1):121119. https://doi.org/10. 1016/j.jece.2026.121119.
  18. Siti Zuraida M, Nurhaslina CR, Hamid KHK. Removal of Synthetic Dyes from Wastewater by Using Bacteria, Lacto-bacillus delbruckii. Int Refereed J Eng Sci. 2013;2(5):1-7.
  19. Gouamid M, Ouahrani MR, Bensaci MB. Adsorption Equilibrium, kinetics and thermodynamics of methylene blue from aqueous solutions using Date Palm Leaves. Energy Procedia. 2013;36:898-907. https://doi.org/10.1016/j.egypro. 2013.07.103.
  20. Eti IA, Khatun M, Khatun MA, Rahman MO, Anis-Ul-Haque KM, Alam MJ. Removal of Dye from Wastewater Using a Novel Composite Film Incorporating Nano-cellulose. Adv Polym Technol. 2023;1-9. https://doi.org/10.1155/2023/443 1941.
  21. Moyo S, Makhanya BP, Zwane PE. Use of bacterial isolates in the treatment of textile dye wastewater: A review. Heliyon. 2022;8(6):e09632. https://doi.org/10.1016/j.heliyon.2022.e09 632.
  22. Sajed TS, Haji A, Khajeh Mehrizi M, Nasiri Boroumand M. Modification of wool protein fiber with plasma and dendrimer: Effects on dyeing with cochineal. Int J Biol Macromol. 2017;107:642-653. https://doi.org/10.1016/j. ijbiomac. 2017.09.038.
  23. Aia L, Zhang Ch, Liao F, Wanga Y, Li M, Meng L, et al. Removal of methylene blue from aqueous solution with magnetite loaded multi-wall carbon nanotube: Kinetic, isotherm and mechanism analysis. J Hazard Mater. 2011; 198:282–290. https://doi.org/10.1016/j.jhazmat.2011.10. 041.
  24. Raj DVK, Renuka Devi M, Venkatesh B, Kubera Sampath Kumar S, Prakash C. Sustainable removal of methylene blue dye from textile effluent by using cellulose nanocrystals extracted from sugarcane bagasse. Biomass Convers Biorefin. 2022;14(10):1-9.https://doi.org/10.1007/s13399-022-03284-5.
  25. Öztürk A, Bayol E, Abdullah MI. Characterization of the biosorption of fast black azo dye K salt by the bacterium Rhodopseudomonas palustris 51ATA strain. Electron J Biotechnol. 2020;46:22-29. https://doi.org/10.1016/j.ejbt. 2020.05. 002.
  26. Schwendler LA, Lansarin MA. Polyhydroxybutyrate-co-hydroxyvalerate/titanium dioxide composite films: Green synthesis, characterization, and photocatalytic assessment. J Polym Res. 2023;30(5):169. https://doi.org/10.1007/s10965-023-03556-2.
  27. Entezari Gh, Otadi M, Amiri R. Study of decolorization ability of orange azo ꞵ-NapHtol by Halomonas Strain Gb bacteria and optimization of the effects of concentration, pH and temperature. Petrol Res. 2015;25:76-87. (In Persian).
  28. Singh S, Chaudhary P, Sharma A. Efficient decolourization of toluidine blue and methylene blue using Bacillus cereus. Vegetos. 2024;37(6):2439-2447. https://doi.org/10.1007/ s42535- 023-00747-6.
  29. Pham VHT, Kim J, Chang S, Chung W. Biodegradation of methylene blue using a novel lignin peroxidase enzyme producing bacteria, named Bacillus sp. React3, as a promising candidate for dye-contaminated wastewater treatment. Fermentation. 2022;8(5):190. https://doi.org/10.3390/ fermen-tation8050190.
  30. Khan I, Saeed K, Zekker I, Zhang B, Hendi AH, Ahmad A, Ahmad S, Zada N, Ahmad H, Shah LA, Shah T, Khan I. Review on methylene blue: Its properties, uses, toxicity and photodegradation. Water. 2022;14(2):242. https://doi.org/10. 3390/w14020242.
  31. Moosavi S, Li RYM, Lai CW, Yusof Y, Gan S, Akbarzadeh O, Chowhury ZZ, Yue XG, Johan MR. Methylene blue dye photocatalytic degradation over synthesised Fe3O4/AC/TiO2 nanocatalyst: Degradation and reusability studies. Nanomater. 2020;10(12):2360. https://doi.org/10.3390/nano 10122360.
  32. Kenawy ER, Shabaka AA, Abou-Zeid AM, Hassouna MS, Elhiti MA. Silica-based nano-adsorbent for enhancement of bacterial biodegradation of methylene blue dye. Desal Water Treat. 2021;241:183-191. https://doi.org/10.5004/dwt.2021. 27829.
  33. Simonin JP. On the comparison of pseudo-first order and pseudo-second order rate laws in the modeling of adsorption kinetics. Chem Eng J. 2016;300:254-263. https://doi.org/10. 1016/j.cej.2016.04.079.
  34. Ullah R, Tuzen M, Hazer B. Synthesized of poly(3-hydroxy butyrate)-b-poly(methyl methacrylate) with Ag nano-particles for removal of methylene blue by multivariate optimization approach. Research Square (Preprint) (2023), https://doi. org/10.21203/rs.3.rs-2450619/v1.
  35. Foo KY, Hameed BH. Insights into the modeling of adsorption isotherm systems. Chem Eng J. 2010;156:2-10. https://doi.org/10.1016/j.cej.2009.09.013.

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