حس‌گر زیستی تهیه شده از نانوالیاف هیبریدی کیتوسان/نانولوله کربنی عامل‌دار به منظور شناسایی نیکوتین

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه مهندسی پزشکی، واحد تهران جنوب، دانشگاه آزاد اسلامی، تهران، ایران، صندوق پستی: 1و2و3 : 1955764955

2 گروه مهندسی پزشکی، واحد تهران جنوب، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

در این تحقیق نیکوتین (NIC) با استفاده از الکترود اصلاح شده کربن شیشه‌ای (GC) به روش ولتامتری چرخه‌ای (CV) شناسایی شده است. سطح الکترود GC با استفاده از روش الکتروریسی و تولید نانو الیاف هیبریدی اصلاح شده است. در این راستا، نانو الیاف هیبریدی از پخش کردن نانولوله کربنی با عامل کربوکسیله (MWCNT-COOH) به عنوان جزء معدنی در بستر پلیمر CS به‌عنوان جزء آلی با شکل منحصر به فرد و ناحیه سطح بالا تولید شده است. میانگین قطر منفذ با افزایش نانولوله کربنی عامل‌دار به‌دلیل افزایش قطر نانوالیاف افزایش یافته است. خواص الکتروشیمیایی NIC با الکترود GC-CS/MWCNT-COOH مورد بررسی قرار گرفت. NIC با الکترود CS/MWCNT-COOH که یک فرآیند کنترل شده با 2 پروتون و 2 الکترون بود کاهش غیرقابل برگشت داشت. سیگنال اکسید شدن در پتانسیل پایین‌تر و جریان‌ بالاتر برای NIC با الکترود اصلاح شده در مقایسه با الکترود GC به دست آمده است که نشان می‌دهد نانولیف حاوی نانولوله کربنی سرعت انتقال الکترون را افزایش می‌دهد. تحت شرایط بهینه، CV اکسید شدن NIC را در 0.82 ولت در محلول بافرفسفات 7.4=pH نشان می‌دهد. منحنی کالیبراسیون خطی محدوده 0.1 تا 100میکرو مولار غلظت NIC (0.9987 ) با حد تشخیص 90 نانومولار را نشان می‌دهد. برای 100 تشخیص موازی 10 میکرومولار NIC برای 5 بار تکرار، 97.2 درصد با انحراف معیار استاندارد 4.08 توانست پایداری خود را نسبت به چرخه اول حفظ نماید که نشان می‌دهد الکترود CS/MWCNT-COOH دارای تکرارپذیری و پایداری عالی است.

کلیدواژه‌ها


عنوان مقاله [English]

Preparation of Bio-Sensor with Hybrid Nanofibers of Chitosan/ Functional Carbon Nanotubes for the Sensing of Nicotine

نویسندگان [English]

  • Abolfazl Mirani 1
  • Laleh Maleknia 1
  • Amir Amirabadi 2
1 Department of Biomedical Engineering, South Tehran Branch, Islamic Azad University, P.O. Box: 1955764955:1,2,3, Tehran, Iran
2 Department of Biomedical Engineering, South Tehran Branch, Islamic Azad University, Tehran Iran
چکیده [English]

In this paper, Nicotine (NIC) was detected by cyclic voltammetry (CV) using a modified glassy carbon electrode (GC). To do this, the surface of the GC electrode was modified by hybrid Nanofiber obtained from electrospinning method. Hybrid nanofibers were produced through the dispersion of carboxylated Multi-walled carbon nanotube (MWCNT-COOH) as an inorganic component in the CS polymer matrix as an organic component. The nanofibers showed unique morpHology and high surface area value. With the increase of functionalized carbon nanotube content in the nanofibers, the mean pore diameter and the average nanofiber diameter increased. The electrochemical properties of nanofibers toward the sensing of NIC were investigated by cyclic voltammetry method. NIC was irreversibly reduced with the use of CS/MWCNT-COOH electrode, a controlled process with 2 protons and 2 electrons. An oxidation signal at lower potential with higher current was obtained for nicotine with the use of polymer-modified electrode compared to glassy carbon electrode.

کلیدواژه‌ها [English]

  • Nicotine
  • Electrochemical Properties
  • nanofiber
  • Chitosan
  • functionalized Multi-walled carbon nanotube
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