Investigation of Methylene Blue Decolorization Using Polyhydroxybutyrate Biopolymer and Bacteria Isolated from Textile Wastewater

Document Type : Original Article

Authors

1 Department of Textile Engineering, Yazd University, P.O. Box: 89195-741, Yazd, Iran

2 Department of Biology, Yazd University, P.O. Box: 89195-741, Yazd, Iran

10.30509/jcst.2026.167761.1281

Abstract

Contamination of textile wastewater with industrial dyes poses a serious environmental threat, and due to the high chemical stability of these dyes, developing efficient removal methods is essential. In this study, two novel bacterial strains (Megaterium SH3 OR635822, and Bacillus cereus SH4 OR636055) and a produced polyhydroxybutyrate (PHB) biofilm were evaluated as bioadsorbents for the removal of the cationic dye methylene blue. Results showed that strain SH4 exhibited the highest dye removal efficiency of 28.45 % under optimal conditions (dye concentration= 100 mg/L, contact time =72 h, pH =7), whereas the PHB biofilm reached 50.81 % under its optimal conditions (dye concentration =100 mg/L, contact time= 60 min, pH =10). Isotherm, kinetic, and thermodynamic analyses indicated that the pseudo-second-order kinetic model and Langmuir isotherm provided the best fit to the experimental data, and the adsorption process was spontaneous and endothermic. This innovative study highlights the high potential of novel bacterial strains and the produced PHB biofilm as efficient bioadsorbents for sustainable treatment of colored wastewater.

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References

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