Detoxification of Dye from Industrial Wastewater by Immobilized Horseradish Peroxidase

Authors

1 Department of Environmental Health, School of Health, Urmia University of Medical Sciences

2 Department of Horticulture, Khoy Branch, Islamic Azad University

3 School of Public Health, Tehran University of Medical Sciences

Abstract

In this study, application of immobilized Horsradish Peroxidase (HRP) on calcium alginate to removal of acid blue 25 and acid orange 7 from waste water was studied. Experimental study of optimum condition for immobilization of HRP on calcium alginate and then, removal efficiency of acid blue 25 and acid orange 7 by immobilized HRP and also toxicity of by-products with bioassay were studied. Results show that immobilized HRP at optimum condition have 80% color removal efficiency from polluted wastewater. Results show alginate beads after 10 replicate usages without change in activity were reusable. Bioassay of by-products by D.Magna show that this method of treatment lead to decrease significantly in toxicity of dye from treated wastewater.

Keywords