کاهش رنگ‌زای آزوی کارموزین از پساب سنتزی مرحله آهارگیری توسط کلبسیلا کوازیپنومونیه GT7

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه مهندسی شیمی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، تهران، ایران، صندوق‌پستی: 775/14515

2 پژوهشکده زیست فناوری صنعت و محیط زیست، پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری، تهران، ایران. صندوق‌پستی: 161/14965

چکیده

حذف رنگ‌زای آزوی کارموزین از محیط پساب سنتزی رنگی که حاوی نشاسته­ بوده و دارای ترکیبی مشابه با خروجی از مرحله آهارگیری بود، توسط باکتری کلبسیلا کوازیپنومونیهGT7 بررسی شد. سنجش غلظت رنگ توسط طیف‌سنجی و اندازه­گیری غلظت نشاسته با استفاده از روش آنترون، نشان داد که انجام فرایند به صورت بی­هوازی در دمای 30 درجه سانتی‌گراد و 7pH ، وقتی که غلظت اولیه کارموزین 50 میلی­گرم بر لیتر می­باشد، طی 48­ساعت منجر به کاهش حدود 96 درصد از غلظت اولیه رنگ‌زا شده است. این، توام با کاهش 30 تا 35 درصد از غلظت اولیه نشاسته (1870 میلی­گرم بر لیتر) بوده است. کروماتوگرافی لایه نازک(TLC) نشان داد که باکتری­ها، رنگ کارموزین حذف شده طی فرایند بی‌هوازی را به آمین­های آروماتیکی سازنده آن تجزیه نموده­اند. ادامه فرایند
به صورت هوازی، با و بدون انجام تلقیح مجدد باکتری­ها در آغاز مرحله هوازی، نشان داد که تا 23روز، شکست ملکول­های آمین آروماتیکی صورت نگرفته و کاهش قابل توجهی در مقدار رنگ یا نشاسته باقی‌مانده از مرحله بی­هوازی، ایجاد نشده است.

کلیدواژه‌ها

عنوان مقاله [English]

The Reduction of Azo Dye Carmoisine from Synthetic Desizing Stage Effluent by Klebsiella quasipneumoniae GT7

نویسندگان [English]

  • Iman Khakbaz 1
  • Tayebe Bagheri Lotfabad 2
  • Amir Heydarinasab 1
  • Pedram Hasanvand 2
1 Department of Chemical Engineering, Science and Research branch, Islamic Azad University, Tehran, Iran
2 Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
چکیده [English]

The removal of the azo dye carmoisine from a synthetic dye wastewater medium, which contained starch and had a similar composition to the effluent from the de-sizing stage, was investigated by Klebsiella quasipneumoniae GT7. The measurements of dye concentration by spectrophotometry and starch concentration by the Anthrone method revealed that the anaerobic process at a temperature of 30 °C and pH 7, with an initial concentration of 50 mg/l of carmoisine, resulted in a decrease of approximately 96 % in the initial dye concentration within 48h. This was accompanied by a decrease of approximately 30 to 35 % in the initial starch concentration (1870 mg/l). Thin layer chromatography (TLC) showed that the bacteria degraded the carmoisine dye, which was removed during the anaerobic process, into its constituent aromatic amines. The continuation of the process under aerobic conditions, with and without re-inoculation of bacteria at the beginning of the aerobic stage, revealed that until day 23, aromatic amine molecules were not degraded and there was no significant reduction in the amount of dye or starch remaining from the anaerobic stage.

کلیدواژه‌ها [English]

  • Dye removal
  • Klebsiella
  • Carmoisine
  • Starch
  • Desizing

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